Fig. 3. PBP-mediated ROS generation is modified by the inhibition of kinases involved in necroptosis. MCF-7 and SKMel-188 cells were cotreated with PBPs at a concentration of 100 μg/ml (a, c) or 200 μg/ml (b, d) and Nec-1 (30 μM), GSK'872 (10 μM) or NSA (1.5 μM). ROS production at 24, 48 and 72 h after stimulation was measured using DCF dye. Data are the means ± SEMs based on values obtained from three independent experiments and expressed in relative fluorescence units.